|
KMID : 0379119980260010127
|
|
Korean Journal of Mycology
1998 Volume.26 No. 1 p.127 ~ p.133
|
|
|
Isolation and Characterization of Regulatory Mutant for Cellulase Production from Trichoderma reesei QM 9414
|
|
|
|
|
Abstract
|
|
|
|
Two regulatory mutants of Trichoderma reesei QM 9414 were isolated by treatment with N-methyl-N´-nitro-N-nitrosoguanidine, and the effects of various inducers on the carboxymethyloellulose (CMC) and filter paper (FP) production were investigated. Induction of CMCase and FPase production of mutants was shown higher level than wild type strain in 1% lactose minimal broth. When induced by glucose, wild type showed glucose-repression for CMCase and FPase production and mutants showed glucose-derepression. Mutant 1 showed 8.38 fold higher CMCase activity and 5.68 fold higher FPase activity than wild type stain. Mutant 2 showed about 8.42 fold higher CMCase activity and 5.41 fold higher FPase activity than wild type strain. Enzyme activities from the mutants and wild type had the same optimum pH of 4.8 and optimum temperature of 60℃.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|